We use an automated robotic system (Q-Bot Genetix) to produce different densities arrays. The Q-Bot uses a 96 or 384 pins head which takes aliquots of bacteria from microplates and spots them in duplicate on nylon membrane (22*22 cm). Using a 2x2 pattern allows the duplicate spotting of 9,216 clones per filter, whereas the 5x5 pattern gathers 27648 clones per filter or 39936 clones with 6*6 pattern.

New : 7*7 Pattern

BAC clones of a library can be spotted using a 7*7 pattern. This density allows decreasing the number of filters to be hybridized in order to perform a screening.

With this pattern it is much more efficient to screen large BAC libraries : less time consuming and lradioactivity consuming.

You can spot 110 592 duplicated BAC clones (144 microplates in duplicate) on a single membrane.

Production of macroarrays is subjected to quality control. A membrane of each batch produced is checked by hybridization using reference probes. All quality insurance procedures are available for our customers on demand.

Macroarray are delivered with a spotting pattern sheme and hybridization protocol.

Applications

Macroarrays are suited for radioactive or non-radioactive hybridization protocols. Each filter can be re-used at least 5 times.

• Colonies filters can be used for qualitative screening experiments (to identify genes of interest, based on information from model plant or related species).

• cDNA filters can be used to perform gene expression analysis experiments.

Please contact us for further details on this service

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