Tomato Genome Sequence

chromosome 7 séquençage

Determining the minimum tilling path from the tomato BAC library in order to sequence chromosome 7

sgn3

In collaboration with:

  •  Dr. Monder Bouzayen
     Laboratoire de Génomique et Biotechnologies des fruits
     UMR990
     Chemin de Borde Rouge
     31326 Castanet Tolosan

Website : http://gbf.ensat.fr/gbf_projects.html

Abstract

The tomato genome will be sequenced as the cornerstone of an International Solanaceae Genome Initiative, a project that aims to develop the family Solanaceae as a model for systems biology for understanding plant adaptation and diversification.

The tomato genome is comprised of approximately 950 Mb of DNA – more than 75% of which is heterochromatin and largely devoid of genes. The majority of genes are found in long contiguous stretches of gene-dense euchromatin located on the distal portions of each chromosome arm. The strategy is to identify and sequence a minimal tiling path of BAC clones through this approximately 220 Mb euchromatin.

The gene-rich euchromatic portion of the tomato genome is being sequenced by an international consortium and the GBF laboratory is leading the French effort for sequencing tomato chromosme 7.

Links: http://www.sgn.cornell.edu/help/about/tomato_sequencing.pl

Results

The first sequencing of the tomato genome has been achieved by the International Tomato Sequencing Consortium and is now available on the SGN web site ( http://solgenomics.net/tomato/).

CNRGV involvement

Responsible : Sonia VAUTRIN

- Macroarray production/ screening
 - 3D DNA pools production
 - DNA pools screening