Plant BAC library construction
Libraries of large genomic DNA inserts are essential to genomics research. The cloning of large genomic DNA fragments into BAC vectors facilitates handling and multiplication as well as long-term conservation. BAC libraries help to identify and isolate genes of interest but also to carry out the physical mapping and sequencing of plant genomes.
We perform whole steps required to produce BAC libraries, from plant tissue to clones ordered in microtiter plates:
- Cell nuclei extraction from frozen material of your plant of interest
- HMW DNA partial digestion
- Sizing of DNA fragments
- Ligation in a vector
- Cloning in phage resistant bactery
- Colony picking using high throughput automated station
- Quality tests and characterization of the library
All steps are tracked via CNRGV's information management system: Genolims.
We propose different strategies according to the level of genomic data available.
Thus if there are no studies on your plant of interest, the BAC library will constitute the gateway to the genes and sequence of interest.
Linked to a genetic map, a BAC library will constitute a reliable basis to establish a physical map and eventually sequence the genome.
If the sequence of the plant genome of your interest or close relative exists, by constructing a BAC library it will be very easy to reveal the genetic diversity of ecotypes or related species of interest.
The number of clones of the BAC library and consequently its coverage will depend on the sequence availability and the goal of your project.
BAC library construction can be associated with the production of 3D-pools or macroarray, their screening and almost any services provided at CNRGV.
Various projects on model or crop plants are already under process.